Fibra-Cel Disks Questions and Answers 2017 by Eppendorf

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Fibra-Cel Disks Questions and Answers 2017 is listed under these categories

Industrial Supply > Laboratory Equipment

Featured catalog pages of Fibra-Cel Disks Questions and Answers 2017

enhanced perfusion new brunswick™ fibra-cel® disks questions and

new brunswick™ fibra-cel® disks questions and answers q what are new brunswick fibra-cel disks a the fibra-cel disks are a solid support material for the growth of mammalian animal and insect cells the disks are used in the packed-bed impeller of new brunswick cell culture bioreactors available in autoclavable sip and single-use platforms animal cells such as hybridoma insect and various anchorage-dependent recombinant cells have all been successfully grown using fibra-cel q why are fibra-cel disks used a the disks are used as a growth matrix for perfusion cultures when secreted products are desired secreted products can be produced either by stably-transformed cell lines or after viral infection the packed-bed impeller design promotes extended perfusion culture lengths for increased productivity the main advantages are low pressure drop across the bed for efficient scale-up higher surface-to-volume ratios less susceptibility to shear forces from impeller blades and sparger

q why are yields with fibra-cel disks superior to that of other supports a a number of cell lines have difficulty attaching to the surface of microcarriers cell lines such as mrc5 often require ideal conditions of temperature in order to attach to the support frequently resulting in reduced cell viability mrc5 as well as most other cell lines attach more readily to the flat surfaces of the fibra-cel disk because they become imbedded in the fiber system and cannot fall off as they do from the spherical surfaces of suspended beads q what causes a reduction in the attachment of cells to microcarriers q what type of toxicity tests are performed a testing for the presence of endotoxins in the material is performed according to usp title xxii chapter 85 page 1493 q why are the fibra-cel disks electrostatically treated a this allows suspension cells such as hybridoma and insect to adhere to the disks and become trapped in the fiber system where they remain throughout the process the

cell lines successfully grown on fibra-cel® disks hybridoma da4.4 123a 127a gamma 67-9-b anchorage-dependent 3t3 cos human osteosarcoma mrc-5 bhk vero cho rcho-tpa rcho hep b surface antigen hek 293 rhek 293 rc127 hep b surface antigen normal human fibroblasts stroma hepatocytes q can fibra-cel disks be used for biomass production insect tn-368 sf 9 rsf 9 hi-5 q are inoculation requirements the same for fibra-cel disks as they are for microcarriers a this application is not recommended since it requires trypsinization for removal of anchorage-dependent cells from the matrix however cells are recoverable from fibra-cel disks for analysis for the growth of anchorage-dependent cells for biomass production the use of microcarriers is frequently preferred suspension cells can be recovered by centrifugation q is it possible to scale up a bioreactor with a fibra-cel disk packed-bed basket insert a with fibra-cel disks requirements are less stringent needing one-third less inoculum